EDUCATION PhD Harvard University November 2007Single Molecule Studies of Enzymatic Dynamic Fluctuations Mentor: Xiaoliang Sunney XieHHMI Janelia Research Campus — Research Scientist — January 2015 – December 2015 HHMI Janelia Research Campus — Research Specialist — January 2013 – December 2014Albert Einstein College of Medicine — Postdoctoral Fellow — September 2010 – December 2012Uppsala University — Postdoctoral Fellow — September 2007 – August 2010Harvard University — Graduate Research Fellow — October 2001 – August 2007Harold A. Scheraga Laboratory — Research Technician — January 2001 – August 2001Cornell University — Chemistry Major — September 1997 – December 2000Fachklinik Ichenhausen — Patient Care Assistant — August 1996 – July 1997HONORS 2015 AAAS Newcomb Cleveland Prize — February 2016 Estonian Science Foundation PUT37 Grant, co-applicant with Dr. Vasili Hauryliuk — August 2012 Postdoctoral Representative to the Einstein Senate — October 2010 – December 2012 Human Frontier Science Program Cross Disciplinary Fellow — June 2008 – August 2011 Young Researcher Participant of the Nobel Laureates Meeting in Lindau — June 2009 Swedish Research Council Postdoctoral Fellowship — January 2008 – May 2008 Student-nominated Fieser Speaker, Harvard Chemistry — April 2007 National Institute of Health Training Grant — January 2002 – July 2005 Eli Lilly Poster Presentation Award, 19th Annual Symposium of the Protein Society — August 2005 George C. Caldwell Prize, Cornell University, Department of Chemistry, Ithaca, NY — October 2001 Phi Beta Kappa Honors Society — May 2001 Bachelor of Arts with Distinction in all Fields (3.96 Cumulative GPA), Ithaca, NY — January 2001 2000 Undergraduate Award in Analytical Chemistry, American Chemical Society — October 2000 ACTIVITIES UT Southwestern — Computational & Systems Biology Seminar Series — November 2017 Multiplexed Single-molecule Live-cell Imaging Reveals the Dynamic Nature of Complex Biological Reactions (pdf) Massachusetts Institute of Technology — Biophysics Seminar — November 2013 Insights into translation by simultaneous single particle tracking of ribosomes and mRNAs Umeå University — International Seminar Series — October 2013 Insights into mRNA translation by simultaneous tracking of ribosomes and mRNAs, and by imaging of cytoskeletal structures in live cells Duke University — Joint Biology and Chemistry Seminar — April 2013 Mechanistic insights from single molecule tracking of individual enzymes, ribosomes and mRNAs in bacteria and mammalian cells University of Tartu — Biomedical Technology Seminar — November 2012 Simultaneous single molecule tracking of β-actin mRNA and the ribosome University of Munich (LMU) — Gene Center Seminar — October 2012 Single molecule investigations into β-actin mRNA localization and compartmentalization University of Munich (LMU) — Invited SFB 594 Seminar — November 2011 Live-cell imaging and single molecule tracking in bacteria and mammalian cells with laser feedback interference and fluorescence microscopy TU Delft — Applied Physics Seminar — April 2009 Stringent Response - From the Test-Tube to Living Cell University of Tartu — Biomedical Technology Seminar — December 2008 Single Molecule Approach to Enzymology - From the Test-Tube to Living Bacterial Cells Harvard University — Student-nominated Fieser Lecture — April 2007 Fluctuating Single Molecules – Zooming in on Enzyme Kinetics Georgia Institute of Technology — Molecular Biophysics Seminar — April 2007 Fluctuating Single Enzyme Molecules Dickinson College — Invited Physics Colloquium — October 2006 Biophysics of Single Molecules - Zooming in on Enzyme Kinetics 4D-Nucleome Annual Meeting, North Bethesda — September 2017 Imaging of Multiple Single-Molecules Reveals the Dynamic Nature of Complex Biological Reactions 10th Berlin Summer Meeting, Computational and Experimental Molecular Biology, Germany — June 2017 Simultaneous Live-Cell Imaging of Multiple Single-Molecules Reveals the Dynamic Nature of Complex Biological Reactions 60th Annual Meeting of the Biophysical Society, Los Angeles, CA — February 2016 Simultaneous High-Speed Tracking of Multiple Single-Molecules Reveals Functional Interactions in Living Cells (abstract) Transcription Imaging Consortium Meeting, HHMI Janelia, Ashburn, VA — September 2015 Tracking multiple single molecules in living cells reveals functional interactions SPIE Optics + Photonics, San Diego, CA — August 2015 A three-camera imaging microscope for high-speed single-molecule tracking and super-resolution imaging in living cells 8th Berlin Summer Meeting, Computational and Experimental Molecular Biology, Germany — June 2015 A three-camera imaging microscope for high-speed single-molecule tracking: Insights into translation by co-movement analysis of ribosomes and mRNAs, and by and super-resolution imaging of cytoskeletal structures in living cellsTranscription Imaging Consortium Meeting, HHMI Janelia, Ashburn, VA — October 2014 A three-color live cell microscope for fast and accurate tracking of multiple interacting molecules EMBO | EMBL Symposium: Seeing is Believing, Heidelberg, Germany — October 2013 Insights into mRNA compartmentalization and translation by simultaneous single particle tracking of ribosomes and mRNAs, and by super-resolution imaging of cytoskeletal structures in live cells Transcription Imaging Consortium Meeting, Janelia Farm, Ashburn, VA — September 2012 Positively alarming: mechanistic insights from single particle tracking in a living cell Focus on Microscopy 2011, Konstanz, Germany — April 2011 Live-cell imaging of invadopodia formation with simultaneous phase-shifted laser feedback interference and fluorescence microscopy Ninth HFSP Awardees Meeting and 20th Anniversary Celebration,Tokyo, Japan — June 2009 Single Molecule Approach to Stringent Response in Individual Living Bacterial Cells 232nd American Chemical Society National Meeting, San Francisco, CA — September 2006 Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited 40th IUPAC World Chemistry Congress, Beijing, China — August 2005 From single molecule enzymology to imaging gene expression in live cells, one molecule at a time CONFERENCE PRESENTATIONS Focus on Microscopy 2015, Göttingen, Germany — April 2015 A three-camera imaging setup and novel cell-permeable dyes for multiplexed single-molecule live cell experiments Focus on Microscopy 2011, Konstanz, Germany — April 2011 Single Molecule Investigations of the Stringent Response Machinery in Living Bacterial Cells 4th Mechanobiology Workshop – Biophysical Society Meeting, Singapore — November 2010 Imaging Adhesions with Phase-Shifted Laser Feedback Interference Microscopy 10th HFSP Awardees Meeting, Thiruvananthapuram, India — November 2010 Stroboscopic Single-molecule Tracking of Freely Diffusing Cytoplasmic Proteins in Living Cells 54th Annual Meeting of the Biophysical Society, San Francisco, CA — February 2010 Single Molecule Tracking Inside Individual Living Bacterial Cells Wallenberg Consortium North Symposium on Modeling and Systems Biology, Stockholm, Sweden —September 2009 Single Molecule Tracking Inside Living Cells Ninth International Conference on Systems Biology, Göteborg, Sweden — August 2008 A Single Molecule Approach to Stringent Response in Individual Living Bacterial Cells 19th Symposium of the Protein Society, Boston, MA — July 2005 A Michaelis-Menten Study of Individual Beta-Galactosidases 228th American Chemical Society National Meeting, Philadelphia, PA — August 2004 Enzymatic dynamics of individual Beta-Galactosidases 17th Symposium of the Protein Society, Boston, MA — July 2003 Single-molecule Enzymatic Dynamics Probed by Fluorescent-product Detection TEACHING FELLOW, HARVARD UNIVERSITY Frontiers in Molecular Biophysics — Fall term, 2003 Principles of Chemistry — Spring term, 2002 Honors Introductory Chemistry — Fall term, 2001 COMPLETED RESEARCH SUPPORT PUT37 (co-applicant, PI: Vasili Hauryliuk) 01/01/2013 – 12/31/2015 Estonian Science Foundation (ETF) ppGpp-mediated activation of RSH proteins: from the mechanism of allosteric regulation to computational properties of the stringent response system Cross Disciplinary Fellow (LT00829/2008, PI: English, Brian P) 06/01/2008 – 08/31/2011 International Human Frontier Science Program (HFSP) Transcription factor dynamics in living bacterial cells at the single cell level Postdoctoral Fellowship (623-2007-8116, PI: English, Brian P) 01/01/2008 – 05/31/2008 Swedish Research Council (VR) The Dynamics of Gene Regulation – The Study of Individual Transcription Factor Molecules in Living Cells Molecular, Cellular and Chemical Biology NIH Training Grant (5 T32 GM07598) 01/01/2002 – 08/31/2005 National Institutes of Health Graduate Research Training Fellowship, Harvard University  |
