Catherine G. Galbraith, Brian P. English, Ulrike Boehm, James A. Galbraith
Compartmentalized Cytoplasmic Flows Direct Protein Transport to the Cell's Leading Edge
bioRxiv. doi: https://doi.org/10.1101/2024.05.12.593794 (May 14, 2024) (open access)
Fadi M. Jradi, Brian P. English, Timothy A. Brown, Jesse Aaron, Satya Khuon, James A. Galbraith, Catherine G. Galbraith, Luke D. Lavis
Coumarin as a general switching auxiliary to prepare photochromic and spontaneously blinking fluorophores
bioRxiv. doi: https://doi.org/10.1101/2024.05.12.593749 (May 12, 2024) (open access)
Katie L. Holland, Sarah E. Plutkis, Timothy A. Daugird, Abhishek Sau, Jonathan B. Grimm, Brian P. English, Qinsi Zheng, Sandeep Dave, Fariha Rahman, Liangqi Xie, Peng Dong, Ariana N. Tkachuk, Timothy A. Brown, Robert H. Singer, Zhe Liu, Catherine G. Galbraith, Siegfried M. Musser, Wesley R. Legant, Luke D. Lavis
A series of spontaneously blinking dyes for super-resolution microscopy
bioRxiv. doi: https://doi.org/10.1101/2024.02.23.581625 (February 4, 2024) (open access)
Yongmei Feng, Stefan Grotegut, Predrag Jovanovic, Valentina Gandin, Steven H. Olson, Rabi Murad, Anne Beall, Sharon Colayco, Paul De-Jesus, Sumit Chanda, Brian P. English, Robert H. Singer, Michael Jackson, Ivan Topisirovic and Ze’ev A. Ronai
Inhibition of coronavirus HCoV-OC43 by targeting the eIF4F complex
Frontiers in Pharmacology 13:1029093. doi: 10.3389/fphar.2022.1029093 (2022) (open access)
Valentina Gandin✶, Brian P. English✶, Melanie Freeman, Louis-Philippe Leroux, Stephan Preibisch, Deepika Walpita, Maritza Jaramillo, Robert H. Singer
Cap-dependent translation initiation monitored in living cells
Nature Communications 13, 6558 (2022) (open access)
Liangqi Xie , Peng Dong , Yifeng Qi , Tsung-Han S. Hsieh , Brian P. English , SeolKyoung Jung , Xingqi Chen , Margherita De Marzio , Rafael Casellas , Howard Y. Chang , Bin Zhang , Robert Tjian, Zhe Liu
BRD2 Compartmentalizes the Accessible Genome
Nature Genetics. doi: https://doi.org/10.1038/s41588-022-01044-9 (2022)
Valentina Gandin✶, Brian P. English✶, Melanie Freeman, Louis-Philippe Leroux, Stephan Preibisch, Deepika Walpita, Maritza Jaramillo, Robert H. Singer
Cap-dependent translation initiation monitored in living cells
bioRxiv. doi: https://doi.org/10.1101/2021.05.21.445166 (May 22, 2021) (open access)
Anand Ranjan, Vu Q. Nguyen, Sheng Liu, Jan Wisniewski, Jee Min Kim, Xiaona Tang, Gaku Mizuguchi, Ejlal Elalaoui, Timothy J. Nickels, Vivian Jou, Brian P. English, Qinsi Zheng, Ed Luk, Luke D. Lavis, Timothee Lionnet, Carl Wu
Live-cell single particle imaging reveals the role of RNA polymerase II in histone H2A.Z eviction
eLife. doi:10.7554/eLife.55667 (April 27, 2020) (open access)
Liangqi Xie, Peng Dong, Xingqi Chen, Tsung-Han S. Hsieh, Sambashiva Banala, Margherita De Marzio, Brian P. English, Yifeng Qi, Seol Kyoung Jung, Kyong-Rim Kieffer-Kwon, Wesley R. Legant, Anders S. Hansen, Anton Schulmann, Rafael Casellas, Bin Zhang, Eric Betzig, Luke D. Lavis, Howard Y. Chang, Robert Tjian✫, Zhe Liu✫
3D ATAC-PALM: Super-resolution Imaging of the Accessible Genome
Nature Methods 17, 430–436 (2020); doi: 10.1038/s41592-020-0775-2
Anand Ranjan, Vu Q. Nguyen, Sheng Liu, Jan Wisniewski, Jee Min Kim, Xiaona Tang, Gaku Mizuguchi, Ejlal Elalaoui, Timothy J. Nickels, Vivian Jou, Brian P. English, Qinsi Zheng, Ed Luk, Luke D. Lavis, Timothee Lionnet, Carl Wu
Live-cell single particle imaging reveals the role of RNA polymerase II in histone H2A.Z eviction
bioRxiv. doi: https://doi.org/10.1101/2020.02.13.947119 (February 13, 2020) (open access)
Liangqi Xie✶, Peng Dong✶, Yifeng Qi, Margherita De Marzio, Xingqi Chen, Sambashiva Banala, Wesley R. Legant, Brian P. English, Anders S. Hansen, Anton Schulmann, Luke D. Lavis, Eric Betzig, Rafael Casellas, Howard Y. Chang, Bin Zhang, Robert Tjian✫, Zhe Liu✫
Super-resolution Imaging Reveals 3D Structure and Organizing Mechanism of Accessible Chromatin
bioRxiv 678649; doi: http://dx.doi.org/10.1101/678649 (June 21, 2019) (open access)
Jonathan B. Grimm, Timothy A. Brown, Brian P. English, Timothée Lionnet, Luke D. Lavis
Synthesis of Janelia Fluor HaloTag and SNAP-tag ligands and their use in cellular imaging experiments
In: Erfle H. (eds) Super-Resolution Microscopy. Methods in Molecular Biology, Vol.1663, Humana Press, New York, NY (2017) (paywall link)
Jonathan B. Grimm✶, Brian P. English✶, Heejun Choi, Anand K. Muthusamy, Brian P. Mehl, Peng Dong, Timothy A. Brown, Jennifer Lippincott-Schwartz, Zhe Liu, Timothée Lionnet✫, Luke D. Lavis✫
Bright photoactivatable fluorophores for single-molecule imaging
Nature Methods 13(12), 985-988 (2016) (paywall link)
Young J. Yoon✶, Bin Wu✶, Adina R Buxbaum, Sulagna Das, Albert Tsai, Brian P. English, Jonathan B. Grimm, Luke D. Lavis, Robert H. Singer
Glutamate-induced RNA localization and translation in neurons
Proceedings of the National Academy of Sciences 113(44), E6877-86 (2016) (open access)
Zhengjian Zhang✫, Brian P. English, Jonathan B. Grimm, Stephanie A. Kazane, Wenxin Hu, Albert Tsai, Carla Inouye, Changjiang You, Jacob Piehler, Peter G. Schultz, Luke D. Lavis, Andrey Revyakin, Robert Tjian
Rapid Dynamics of General Transcription Factor TFIIB Binding During Preinitiation Complex Assembly Revealed by Single-Molecule Analysis
Genes and Development 30, 2106-2118 (2016) (open access)
Luke D. Lavis✫, Jonathan B. Grimm, Brian P. English, Heejun Choi, Anand K. Muthusamy, Brian P. Mehl, Peng Dong, Timothy A. Brown, Jennifer Lippincott-Schwartz, Zhe Liu, Timothée Lionnet
Bright photoactivatable fluorophores for single-molecule imaging
bioRxiv 066779; doi: http://dx.doi.org/10.1101/066779 (July 29, 2016) (open access)
Paul W. Tillberg✶, Fei Chen✶, Kiryl D. Piatkevich, Yongxin Zhao, Chih-Chieh (Jay) Yu, Brian P. English, Linyi Gao, Anthony Martorell, Ho-Jun Suk, Fumiaki Yoshida, Ellen M. DeGennaro, Douglas H. Roossien, Guanyu Gong, Uthpala Seneviratne, Steven R. Tannenbaum, Robert Desimone, Dawen Cai, Edward S. Boyden
Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies
Nature Biotechnology 34, 987–992 (2016) (paywall link) (cover legend)
Tatsuya Morisaki, Kenneth Lyon, Keith F. DeLuca, Jennifer G. DeLuca, Brian P. English, Zhengjian Zhang, Luke D. Lavis, Jonathan B. Grimm, Sarada Viswanathan, Loren L. Looger, Timothée Lionnet, Timothy J. Stasevich
Real-time quantification of single RNA translation dynamics in living cells
Science 352(6292), 1425-1429 (2016) (paywall link)
Won-Ki Cho, Namrata Jayanth, Brian P. English, Takuma Inoue, J. Owen Andrews, William Conway, Jonathan B. Grimm, Jan-Hendrik Spille, Luke D. Lavis, Timothée Lionnet✫, Ibrahim I Cissé✫
RNA Polymerase II cluster dynamics predict mRNA output in living cells
eLife 2016;10.7554/eLife.13617 (May 3, 2016) (open access)
Zachary B. Katz✶, Brian P. English✶, Timothée Lionnet, Young J. Yoon, Nilah Monnier, Ben Ovryn, Mark Bathe, Robert H. Singer
Mapping translation “hotspots” in live cells by tracking single molecules of mRNA and ribosomes
eLife 2016;5:e10415 (January 13, 2016) (open access) (pdf)
Brian P. English, Robert H. Singer
Tracking multiple single molecules in living cells
SPIE Newsroom 10.1117/2.1201509.006125 (November 2, 2015) (open access) (pdf)
Brian P. English✫, Robert H. Singer
A three-camera imaging microscope for high-speed single-molecule tracking and super-resolution imaging in living cells
Proc. SPIE 9550, Biosensing and Nanomedicine VIII, 955008 (invited paper) 10.1117/12.2190246 (2015) (free PMC article) (pdf)
Nilah Monnier, Zachary Barry, Hye Yoon Park, Kuan-Chung Su, Zachary Katz, Brian P. English, Arkajit Dey, Keyao Pan, Iain M. Cheeseman, Robert H. Singer, Mark Bathe
Inferring transient particle transport dynamics in live cells
Nature Methods 12(9), 838–840 (2015) (free PMC article)
Sarada Viswanathan, Megan E. Williams, Erik B. Bloss, Timothy J. Stasevich, Colenso M. Speer, Aljoscha Nern, Barret D. Pfeiffer, Bryan M. Hooks, Wei-Ping Li, Brian P. English, Teresa Tian, Gilbert L. Henry, John J. Macklin, Ronak Patel, Charles R. Gerfen, Xiaowei Zhuang, Yalin Wang, Gerald M. Rubin, Loren L. Looger
High-performance probes for light and electron microscopy
Nature Methods 12(6), 568–576 (2015) (free PMC article)
Johnathan B Grimm, Brian P English, Jiji Chen, Joel P Slaughter, Zhengjian Zhang, Andrey Revyakin, Ronak Patel, John J Macklin, Davide Normanno, Robert H Singer, Timothée Lionnet✫, Luke D Lavis✫
A general method to improve fluorophores for live-cell and single-molecule microscopy
Nature Methods 12(3), 244-250 (2015) (free PMC article) (cover legend)
Highlighted in: Select, New Antibiotic, Unexpected Antiviral, IP7 in Cancer, and Better Dyes, Milka Kostic, Chemistry and Biology 22(2), 159-160 (2015) (paywall link)
Jiang Hao✶, Brian P English✶, Rachel Hazan, Peng Wu✫, Ben Ovryn✫
Tracking surface glycans on live cancer cells with single molecule sensitivity
Angewandte Chemie International Edition 54(6), 1765–1769 (2015) (free PMC article)
Highlighted in:
C&EN News: Single Glycoproteins Caught In Motion 93(1), 21 (2015) (paywall link)
Bi-Chang Chen✶, Wesley R Legant✶, Kai Wang✶, Lin Shao, Daniel E Milkie, Michael W Davidson, Chris Janetopoulos, Xufeng S Wu, John A Hammer III, Zhe Liu, Brian P English, Yuko Mimori-Kiyosue, Daniel P Romero, Alex T Ritter, Jennifer Lippincott-Schwartz, Lillian Fritz-Laylin, R Dyche Mullins, Diana M Mitchell, Joshua N Bembenek, Anne-Cecile Reymann, Ralph Böhme, Stephan W Grill, Jennifer T Wang, Geraldine Seydoux, U Serdar Tulu, Daniel P Kiehart, Eric Betzig
Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
Science 346(6208), 1257998 (2014) (free PMC article) (cover legend)
Kiryl D Piatkevich, Brian P English, Vladimir N Malashkevich, Hui Xiao, Steven C Almo, Robert H Singer & Vladislav V Verkhusha
Photoswitchable Red Fluorescent Protein with a Large Stokes Shift
Chemistry & Biology 21, 1402–1414 (2014) (open access)
Viktoriya Shyp, Stoyan Tankov, Andrey Ermakov, Pavel Kudrin, Brian P English, Måns Ehrenberg, Tanel Tenson, Johan Elf & Vasili Hauryliuk
Positive allosteric feedback regulation of the stringent response enzyme RelA by its product
EMBO Reports 13(9), 835-839 (2012) (open access)
Discussed in:
Research Highlights, Positively alarming, Catherine Goodman, Nature Chemical Biology 8 (2012) (link)
Anton Kuzemenko✶, Stoyan Tankov✶, Brian P English✶, Ivan Tarassov, Tanel Tenson, Piotr Kamenski, Johan Elf & Vasili Hauryliuk
Single molecule tracking fluorescence microscopy in mitochondria reveals highly dynamic but confined movement of Tom40
Scientific Reports 1, 195; 10.1038/srep00195 (2011) (open access) (SGD curated paper)
Brian P English, Vasili Hauryliuk✶, Arash Sanamrad✶, Stoyan Tankov, Nynke Dekker & Johan Elf
Single Molecule Investigations of the Stringent Response Machinery in Living Bacterial Cells
Proceedings of the National Academy of Sciences 108(31), E365-73 (2011) (open access)
Discussed in:
Research Highlights, Proteins in Motion, Michael Eisenstein, Biopolymers 95 (2011) (pdf)
Signalling in space and time: systems dynamics of intracellular communication, Shelly Tzlil & Rejji Kuruvilla, EMBO reports 12 (2011) (link)
In vitro and in vivo single-molecule fluorescence imaging of ribosome-catalyzed protein synthesis, Corey E Perez & Ruben L Gonzalez Jr, Current Opinion in Chemical Biology 15 (2011) (paywall link)
Featured on the HFSP homepage (link)
Brian P English✶, Arash Sanamrad✶, Stoyan Tankov, Vasili Hauryliuk & Johan Elf
Tracking of individual freely diffusing fluorescent protein molecules in the bacterial cytoplasm
arXiv, 1003.2110v1 [q-bio.QM] (2010) (pdf)
Brian P English, Wei Min, Antoine M van Oijen, Kang Taek Lee, Guobin Luo, Hongye Sun, Binny J Cherayil, S C Kou & X Sunney Xie
Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited
Nature Chemical Biology 2, 87-94 (2006) (paywall link) (pdf)
Discussed in:
Michaelis-Menten is dead, long live Michaelis-Menten!, Nils G. Walter, Nature Chemical Biology 2 (2006) (pdf)
Michaelis-Menten for single enzyme molecules, Allison Doerr, Nature Methods 3 (2006) (pdf)
Physics Update: Zooming in on Enzyme Kinetics, Stephen G. Benka, Physics Today (April, 2006) (pdf)
Wei Min, Irina V Gopich, Brian P English, S C Kou, X Sunney Xie & Attila Szabo
When does the Michaelis-Menten equation hold for fluctuating enzymes?
The Journal of Physical Chemistry B 110, 20093-20097 (2006) (paywall link) (pdf)
S C Kou, Binny J Cherayil, Wei Min, Brian P English & X Sunney Xie
Single-Molecule Michaelis-Menten Equations
The Journal of Physical Chemistry B 109, 19068-19081 (2005)
(paywall link) (cover legend) (pdf)
Wei Min, Brian P English, Guobin Luo, Binny J Cherayil, S C Kou & X Sunney Xie
Fluctuating Enzymes: Lessons from Single-Molecule Studies
Accounts of Chemical Research 38, 923-931 (2005) (paywall link) (pdf)
Brian P English, Ervin Welker, Mahesh Narayan & Harold A Scheraga
Development of a Novel Method To Populate Native Disulfide-Bonded Intermediates for Structural Characterization of Proteins: Implications for the Mechanism of Oxidative Folding of RNase A
Journal of the American Chemical Society 124, 4995-4999 (2002) (paywall link) (pdf)
